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Online ISSN: 2278-1404

International Journal of Fundamental and Applied Sciences

Cytotoxic potential of a pigment from Serratia marcescens against HepG2 and Jurkat cell lines and optimization of culture condi tions for enhanced pigment production


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Year 2016 Vol 5 Issue 1
Document Type : ORIGINAL ARTICLE
1Megha Shukla 2Varalakshmi K. N.
1Department of Biotechnology, Centre for Post-Gradua te Studies, Jain University, Bangalore – 560 011, K arnataka, INDIA
2Department of Biotechnology, Centre for Post-Gradua te Studies, Jain University, Bangalore – 560 011, K arnataka, INDIA
Abstract
Pigment from micro-organism is one of the emerging areas of research and many pigments have been extensively studied for their therapeutic potential.

Our aim was to analyze the anticancer property of a red pigment extracted from Serratia marcescens JGI 27against HepG2 and Jurkat cell lines. The cel l viability was evaluated by MTT assay. As the red pigment extracte d from S. marcescens exhibited high cytotoxicity to the tested cancer cell lines, the pigment production was optimized under various culture conditions like, temperature shock, incubation time, carbon sources, nitrogen sources and metal ions. Pigment yield was analyzed spectrophotometrically at 487 nm.

Keywords
Cytotoxicity, HepG2 cells, Jurkat cells, CHO cells, Serretia marcescens.
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