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Online ISSN: 2278-1404

International Journal of Fundamental and Applied Sciences

Genome-Wide Analysis of Conserved Orthologous Synteny: A Strategic Approach to Discover COPD Drug Targets


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Year 2018 Vol 7 Issue 4
Document Type : ORIGINAL ARTICLE
1Ravindranath B S
1Dept. of Biotechnology, Manipal Institute of Technology, Eshwarnagar, Manipal
Abstract

The principal objective of this study was to identify the drug targets based on synteny in the pathogenomes and to prioritize the potential COPD therapeutic targets.
Comparison of the patho-genomes based on synteny was successfully executed by employing SynteView standalone tool continued by classification of orthologous genes. Further, the classified orthologous genes were searched against the Human genome for the sequence similarity, essentiality analysis was achieved by BLAST analysis, protein-protein interactions were predicted based on STRING analysis. Toxicity and druggability were predicted and classification of the class of drug targets was performed for the prioritization of the potential drug targets. Results: Use of Synteny based comparative genomics strategy was directional in the identification of putative therapeutic targets in Chlamydophila pneumoniae, which led to classify 1128 syntons into orthologous and non-orthologous syntons.
639 syntons were highly conserved in all the Chlamydial organisms. 414 proteins the as non-host, 57 proteins predicted as essential, subcellular localization analysis revealed 19 cytoplasmic proteins, two outer membrane proteins, and 1 extracellular protein, further functional classification led to the prediction of transmembranes, lipoproteins and signal peptides which yielded four transmembranes, four signal peptides, three Lipoproteins, and 1 Transferase. DNA-directed RNA polymerase subunit beta was as putative potential drug target based on orthologous syntons. Conclusion: Synteny based comparison of multiple pathogenic genomes is promising in identifying conserved orthologous genes which were crucial in predicting the drug targets strategically and prioritize DNA-directed RNA polymerase subunit beta and ribonucleotide diphosphate reductase subunit alpha as the potential COPD drug targets.
Keywords
Synteny blocks, Chlamydiaceae, Chlamydophila pneumoniae, patho-genome, orthologous, Comparative genomics, COPD, therapeutic targets
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