International Journal of Fundamental and Applied Sciences
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(ICV-Poland) Impact Value: 5.62

Online ISSN: 2278-1404

International Journal of Fundamental and Applied Sciences

Determination of genetic fidelity of Micropropagate d plants of Zingiber officinale cv- Majhauley of Sikkim Himalaya using RAPD markers


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Year 2012 Vol 1 Issue 2
Document Type : Original Article
1Bharat C. Basistha 2 2Pratap J. Handique 1 3Sushen Pradhan 1*
12 Sikkim State Council of Science and Technology, Gang tok, Sikkim, India
21 Department of Biotechnology, Gauhati University, Guw ahati – 781014, Assam, India
31 Department of Biotechnology, Gauhati University, Guw ahati – 781014, Assam, India
Abstract
The present investigation was carried out for the d etermination of genetic conformity of micropropagat ed plants of Zingiber officinale cv Majhauley using Random amplified polymorphic DNA (RAPD) techn ique.

.Total fifteen different arbitrary decamers were used as primers to amplify DNA from in vitro plant material to assess the genetic fidelity of 1 8 months old in vitro cultured plantlets.

Keywords
Zingiber officinale, Majhauley, fidelity, RAPD, Sikkim
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