International Journal of Fundamental and Applied Sciences
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Online ISSN: 2278-1404

International Journal of Fundamental and Applied Sciences

In vitro antioxidant profiling of Bambusa tulda Roxb. aqueous methanolic leaf extract growing in the forests of Kokrajhar district, BTAD, Assam, India


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Year 2015 Vol 4 Issue 3
Document Type : ORIGINAL ARTICLE
1Swarna Kamal Dey1, 2Birendra Kumar Brahma1 and Arvind Kumar Goyal 3Arvind Kumar Goyal 4Sushil Kumar Middha2, 5Talambedu Usha3,
11Bamboo Technology, Department of Biotechnology, Bodoland University, Kokrajhar-783370, B.T.A.D, Assam, India
23Department of Biochemistry, MLA College for Women, Bangalore-560012, Karnataka, India
31Bamboo Technology, Department of Biotechnology, Bodoland University, Kokrajhar-783370, B.T.A.D, Assam, India
42Department of Biotechnology, MLA College for Women, Bangalore-560012, Karnataka, India
52Department of Biotechnology, MLA College for Women, Bangalore-560012, Karnataka, India
Abstract
From an age-old period bamboo has been an important ingredient of traditional Asian medicines in general and Chinese medicine in particular. Today people are searching over the use of herbal medicine instead of the synthetic drugs. Bambusa tulda (Poaceae) is widely distributed in North Eastern parts of the Country.
The aim of this study was to evaluate phytochemical, biochemical constituent and in vitro antioxidant activity of aqueous methanolic leaf extract of Bambusa tulda.
Phytochemical constituents of leaves were extracted by hot extraction method.Aqueous methanolic extract showed that the presence of phenolic compound, alkaloids, saponnins, flavonoids, steroids, anthraquinones, glycosides. The total phenolic, flavonoid and flavonol content in the aqueous methanolic extract were determined by calorimetric assay as well as their antioxidant activity through various chemical assays like DPPH radicals, hydrogen peroxide scavenging and reducing power assay.
The total phenolic, flavonoids and flavonol content in aqueous methanolic extract were 17.494±0.01 mg GAE/g, 176.35±0.03 mg QE /g and 96.2±0.01 mg QE /g respectively. Conclusion: This may be first report to provide evidence that the crude aqueous metanolic extract of Bambusa tulda leaf is a potential source of natural antioxidant.
Keywords
Bambusa tulda antioxidants DPPH, Kokrajhar Jati banh correlation FRP, phenolics, Owa gubwai
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