Cytotoxic potential of a pigment from Serratia marcescens against HepG2 and Jurkat cell lines and optimization of culture condi tions for enhanced pigment production
PDF (203 KB) Year 2016 Vol 5 Issue 1 Document Type : ORIGINAL ARTICLE
1Megha Shukla 2Varalakshmi K. N. 3c 1Department of Biotechnology, Centre for Post-Gradua te Studies, Jain University, Bangalore – 560 011, K arnataka, INDIA
2Department of Biotechnology, Centre for Post-Gradua te Studies, Jain University, Bangalore – 560 011, K arnataka, INDIA
3v
AbstractPigment from micro-organism is one of the emerging
areas of research and many pigments have been
extensively studied for their therapeutic potential.Our aim was to analyze the anticancer property of
a
red pigment extracted from
Serratia marcescens
JGI 27against HepG2 and Jurkat cell lines. The cel
l viability was
evaluated by MTT assay. As the red pigment extracte
d from
S. marcescens
exhibited high cytotoxicity to the tested
cancer cell lines, the pigment production was optimized under various culture conditions like, temperature shock, incubation time, carbon sources, nitrogen sources and metal ions. Pigment yield was analyzed spectrophotometrically
at 487 nm.
KeywordsCytotoxicity, HepG2 cells, Jurkat cells, CHO cells,
Serretia marcescens. StatisticsArticle View:PDF Download:3013XML Download:1368