Subtyping of HIV - 1 BY gag heteroduplex mobility assay
PDF (952 KB) Year 2015 Vol 4 Issue 2 Document Type : original article
1Santanu Das 1 2 Srikanth Tripathy 2 11 Department of Biotechnology, Seshadripuram First Grade College, Yelahanka New Town, Bangalore, Karnataka
2Molecular Virology National AIDS Research Institute (ICMR) Pune, Maharashtra, India
AbstractSurveillance of the different HIV
-
1 subtypes has important implications for developing candidate
vaccines and understanding the dynamics of HIV
-
1 transmission in various populations. In this study, HIV
-
1 viral subtypes were
determined for homologies in p24 region by the heteroduplex mobility assay (HMA) in 124 patients and by sequencing of ‘
gag
gene’ with sexually transmitted diseases (STD) in selected places all over IndiaProviral DNA from whole blood
and from PBMC’s from recent sero
-
converters and seropositive individuals were analyzed.
Of 121 samples analyzed, 115
(95%) were HIV
-
1 C subtype, 5(4%) were HIV
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1 A subtype and 1(1%) was HIV
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1 B subtype. Further analysis revealed that 114
(99%) of the C subtype samples had maximum homology to the C6
-
Indian reference strain, while 1 (1%) was most homologous to
the C3
-
Zambian strain.
Conclusion:
These findings have important implications for the design and testing of effective candidate
HIV
-
1 vaccines in India.
Keywords
HIV
-
1 heteroduplex mobility assay AIDS STDStatisticsArticle View:PDF Download:1790XML Download:824