Phytochemical Screening and Biological Activities of Impatiens balsamina. L seeds

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Year 2014 Vol 3 Issue 2
Document Type : Original article
¹Shivakumara, ² Seitjit Wahengbam ³ Naveen Kumar Rana ⁴ Swarnava Kundu, ⁵ Shivaji Bole* ⁶Vedamurthy AB
¹Department of Biotechnology, The Oxford College of Science, Bangalore-78, India
²Department of Biotechnology, The Oxford College of Science, Bangalore-78, India
³Department of Biotechnology, The Oxford College of Science, Bangalore-78, India
⁴Department of Biotechnology, The Oxford College of Science, Bangalore-78, India
⁵Department of Biotechnology, The Oxford College of Science, Bangalore-78, India
⁶Department of Biotechnology, The Oxford College of Science, Bangalore-78, India
Abstract
In the present study, seeds of Impatiens balsamina L are subjected to phytochemical evaluation and biological activities like, antioxidant, antidiabetic and antiinflamatory studies in-vitro

Antioxidant activity is studied using reducing power assay and phospho molybdenum assay, the results were compared with standard ascorbic acid. Free radical scavenging assay % of inhibition is calculated. Antidiabetic and Anti inflammatory activity were calculated by Amylase inhibition and BSA denaturation assay respectively.
Phytochemical screening of the ethanol extract of seeds revealed the presence of alkaloids, flavanoids, terpenoids, tannins and saponins. IC50 value of extract is found to be 320µg/ml. , Seed extract exhibited significant anti-diabetic activity as compared to standard drug in-vitro. For invitro studies for anti-inflammatory assay, IC50 value was found to be 210 µg/ml. The study demonstrates significant analgesic and anti-inflammatory effects of aqueous extract of Impatiens balsamina leaves.
Keywords
Impatiens balsamina L., anti-inflammatory, anti diabetic, IC50
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