Aluminum Induced Biochemical Alterations in Amaranthus tricolor

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Year 2013 Vol 2 Issue 4
Document Type : Original article
¹Bhanuprakash KS ² Sumit Jha ³ Niraj Jha ⁴Moumita Sardar ⁵ Harika Annapragada ⁶ Devika Mohan T ⁷Prakash A Bapodara ⁸ Sangita Roy*
¹Department of Biochemistry, The Oxford College of Science, Bangalore 560102, Karnataka, India.
²Department of Biochemistry, The Oxford College of Science, Bangalore 560102, Karnataka, India.
³Department of Biochemistry, The Oxford College of Science, Bangalore 560102, Karnataka, India.
⁴Department of Biochemistry, The Oxford College of Science, Bangalore 560102, Karnataka, India.
⁵Department of Biochemistry, The Oxford College of Science, Bangalore 560102, Karnataka, India.
⁶Department of Biochemistry, The Oxford College of Science, Bangalore 560102, Karnataka, India.
⁷Department of Biochemistry, The Oxford College of Science, Bangalore 560102, Karnataka, India.
⁸Department of Biochemistry, The Oxford College of Science, Bangalore 560102, Karnataka, India.
Abstract
The current study reveals the effect of Aluminum (Al) on Amaranthus tricolor L and subsequent biochemical changes on the plant. In this study, the effect of Al stress on growth and other related antioxidants and enzymes were investigated.

This was achieved by investigating plant growth parameters (root/shoot length, dry weight) in presence and absence of various concentrations of Al. The antioxidant activity was determined by estimating proline, ascorbate. The effects of scavenging enzymes were determined by Catalase, Glutathion and SOD activity. The accumulation of Al in root and shoot was determined by FAAS (Flame Atomic Absorption Spectroscopy).
Al induced growth retardation observed more in roots (52.2%) compared to shoots (35.3%). Total concentration of proline increased (18 folds) compared to the control. The antioxidants and enzymes exhibited initial increase and subsequent decrease in response to Al stress. Accumulation of Al (by FAAS) was predominantly in root (70.44ppm ± 0.420).Result thus suggested Al induced growth inhibition in Amaranthus tricolor and the primary affected region is the root. Al accumulation in the roots could play an important role in the acclimation of the genotype to Al stress, and could be used as physiological markers for Altolerance. Metal induced scavenging mechanism observed both at enzymatic and antioxidant molecular level.
Keywords
Amaranthus tricolor, Aluminum, Antioxidant, ROS, Scavenging enzyme
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